PLX297478

GSE101415: Intestinal IKKa is required for tumor initiation in APC mutant mice

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

We have generated a mouse model for tumor initiation carrying a mutation in APC and lacking IKK in intestinal epithelial cells. IKK-deficient intestinal cells primarily failed to generate adenomas, and the few adenomas arising in this background displayed a significant reduction in cell proliferation. Using an in vitro model for intestinal tumoroids (derived from adenoma initiating cells), we have performed RNA sequencing of wild type and IKK-deficient intestinal tumoroids. This has demonstrated that epithelial IKK controls transcription of stem cell-related genes and genes associated with proliferation and apoptosis. SOURCE: Lluis Espinosa (lespinosa@imim.es) - Stem Cell and Cancer Hospital del Mar