PLX297265

GSE106365: The transcriptome difference analysis after overexpression of lncRNA-smad7 in skeletal muscle damage

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Long noncoding RNAs (lncRNAs) have been demonstrated to play important roles in skeletal muscle development. However, their regulation remains poorly understood. In the present study, to screen RNA-seq data about lncRNAs in myoblasts and myotubes, we selected a difference expression lncRNA, lncRNA-smad7. We aimed to investigate the function and molecular mechanism of lncRNA-smad7 during skeletal muscle development. Functional studies had shown that lncRNA-smad7 promoted myoblasts G1 phase arrest by flow cytometry and myoblasts differentiation by qRT-PCR, western blot and immunofluorescence in vitro. LncRNA-smad7 also promoted skeletal muscle regeneration in Cardiotoxin (CTX) induced muscle injury in vivo, which was mainly elucidated by HE staining and RNA-seq analysis, etc. Further mechanism research indicated that lncRNA-smad7 could increase smad7 and IGF2 protein expression level. Whereas, overexpression of miR-125b inhibited smad7 and IGF2 protein expression as well as lncRNA-smad7 and smad7 3UTR luciferase activity, which were validated with a dual luciferase report system. In summary, the functional role and molecular mechanism manifested that lncRNA-smad7 promoted myoblasts differentiation and skeletal muscle regeneration through sponging miR-125b. SOURCE: Chengchuang Song (chengchuangsong@163.com) - Northwest A&F University