Key Features
Enhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreThe role of chromatin-mediated regulation in cell-fate specification during mammalian development is been increasingly appreciated. However, very little is known about epigenetic regulatory networks during embryonic neurogenesis. In search for novel epigenetic regulators of neuronal development, we find that Phf21b is specifically expressed in the cortex during embryonic neurogenesis. Interestingly, knockdown of Phf21b during cortical development leads to a defective neurogenesis. Immunoprecipitation assay combined with mass-spectrometry revealed that Phf21b associates with the repressive chromatin machinery including histone deacetylases as well as Lsd1, suggesting that it likely functions in gene repression. Biochemical analysis revealed that Phf21b has higher affinity for H3K4me1 compared to other modifications states of histone H3 at Lysine 4 (K4). Our data suggests that Phf21b targets the regulatory regions of the cell cycle genes where they recruit Lsd1 and Hdac2 to repress them via loss of H3K4me1 and H3K27ac. Overall, we identify Phf21b as a novel essential regulator of neurogenesis, which mediates epigenetic silencing of cell cycle genes during cortical development. SOURCE: Hyobin Jeong (h.jeong@imb-mainz.de) - IMB
View on GEOView in PlutoEnhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreUse Pluto's intuitive interface to analyze and visualize data for this experiment. Pluto's platform is equipped with an API & SDKs, making it easy to integrate into your internal bioinformatics processes.
Read about post-pipeline analysisView quality control data and experiment metadata for this experiment.
Request imports from GEO or TCGA directly within Pluto Bio.
Chat with our Scientific Insights team