PLX079440

GSE109472: p110 of PI3K is Indispensable for Quiescence Exit in Adult Muscle Satellite Cells

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Adult muscle stem cells (MuSC) are quiescent with a localization between myofibers and basal lamina. Upon injury, MuSC exit quiescence, reenter cell cycle, expand and differentiate for muscle regeneration. By using genetic mouse model, we identified p110/mTORC1 signaling as a indispensable pathway that permits quiescence exit and cell cycle reentry. In order to dig out the downstream effectors, we compared the transcriptome of freshly isolated MuSC from Ctrl (p110-f/+:R26-YFP/YFP:Pax7-CreER/CreER) to MuSC-specific p110-null (iKO, p110-f/f:R26-YFP/YFP:Pax7-CreER/CreER) mice by RNA-sequencing, and AP1 target genes were dramatically down-regulated in iKO MuSC. Restoration of Jun could significantly rescue the cell cycle reentry defect in iKO MuSC. In summary, we provided a p110/mTORC1/Jun axis required for quiecence exit and cell cycle reentry of MuSC. SOURCE: Han ZHU (denniszhu20@gmail.com) - WU Zhenguo Lab HKUST