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Learn MoreWe used RNA-seq to characterize the contributions of PBRM1 to gene expression in HK-2, NMuMG, and Caki1 cell lines. Further, we used ATAC-seq to characterize the effects of PBRM1 on chromatin accessibility in HK-2 and NMuMG cell lines. Finally, we used RNA-seq to determine the gene expression profile of Caki2 cells grown under 3D culture conditions with and without PBRM1. Combined, our analyses reveal a role for PBRM1 in expression of genes associated with stress responses and with specific transcription factor families, such as the FOXO family. SOURCE: Benjamin Carter (beccarte@gmail.com) - Keji Zhao National Institutes of Health
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