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Learn MorePurpose: To identify the in vivo RNA targets of PCIF1, we performed meRIP experiments for PCIF1 KD and control cells using an anti-m6A antibody. We envisioned that the cap-specific PCIF1 should selectively alter the m6Am modification at the 5-terminal region of transcripts. Indeed, we observed a reduction of modification peaks at the 5-end but not the 3-UTR regions of mRNA upon PCIF1 KD. SOURCE: Kai Li (li_kai@pku.edu.cn) - Yi Lab Peking University
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