PLX038956

GSE122781: RNA sequencing of mutant AKT tumors

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Goal: Comparison of mRNA expression profiles in primary tumors based on activation of different AKT paralogs; Methods: RNA sequencing performed at the High-Throughput Genomics and Bioinformatics Analysis Core at the University of Utah. Detailed procedures available in methods and DNA processing pipline sections.; Results: Focus of study compared mRNA expression profiles of BRAFV600E;Cdkn2a-/-;Pten-/- and BRAFV600E;Cdkn2a-/-;Pten-/-;AKT1E17K cohorts. PCA and unsupervised heirarchical clustering revealed samples clustered together appropriately. Seventeen of eighteen FA factor genes analyzed were upregulated in the AKT1E17K cohort.; Conclusion: AKT1E17K has a critical role in upregulating a large group of FA factors SOURCE: Sheri Holmen (sheri.holmen@hci.utah.edu) - University of Utah