PLX165019

GSE123022: Deep single-cell RNAseq of postnatal day 7 microglia from wild type and Trem2 knockout brains

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

We generated single-cell RNAseq profiles of 369 microglia (183 from wild type and 186 from Trem2 knock-out), sorted in the gate CD45lowCD11+ or CD45lowCD11+Gpnmb+Clec7a+ (PAM enrichment), to compare gene expression of wild type vs. Trem2 knock-out microglia on the postnatal day 7. Single cells were FACS index sorted from the whole brain followed by Smart-seq2 library preparation and Illumina Nextseq (sequence depth > 1 million per cell). A total of 334 cells passed quality control for data analysis. Microglia in the Trem2 knock-out contained a similar PAM population with characteristic gene expression, suggesting that the presence of early postnatal PAM do not depend on TREM2. SOURCE: Qingyun Li (tristan.qingyun.li@gmail.com) - Stanford University