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PLX273700
GSE128263: Cadmium exposure induces SNAIL1 expression via downregulation of miR-30e in human bronchial epithelial cells
- Organsim human
- Type RNASEQ
- Target gene
- Project ARCHS4
Purpose: To understand the molecular mechanisms underlying Cd exposure-induced diseases.; Methods: Immortalized human bronchial epithelial cells (BEAS-2B) were cultured in Dulbeccos Modified Eagles Medium (DMEM, Cellgro) supplemented with 1% Penicillin Streptomycin and 10% Fetal Bovine Serum (FBS, Atlanta Biologicals) at 37 degree C and 5 % CO2. For Cd exposure, 0, 2.5, 5 and 10 M of CdCl2 was added to the media and the cells were cultured for 72h.; Results: This study shows that cadmium exposure induces SNAIL1 expression via miR-30e downregulation and the cells undergo epithelial-mesenchymal transition. SOURCE: Suresh Cuddapah (suresh.cuddapah@nyulangone.org) - Suresh Cuddapah NYU-Langone Health
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