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Learn MoreBlood samples were collected from all participants in 10-mL EDTA-coated Vacutainer tubes. Plasma was separated by centrifugation at 3,000 rpm for 10 min at room temperature (25C) within 2 h after blood collection and then centrifuged at 13,000 rpm for 10 min at 4C to remove debris. Isolated plasma samples were stored at -80C until use. EV RNA were isolated by affinity-based binding to spin columns using an exoRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany). RNA libraries were prepared for sequencing using SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. RNA-seq was performed by the Illumina sequencing platform on a 150-bp paired-end run. SOURCE: Shenglin Huang Fudan University
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