Key Features
Enhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreDespite the importance of germ cell differentiation for sexual reproduction, gene networks underlying their fate remain unclear. Here, we describe a comprehensive characterization of gene expression dynamics during sex determination based on single-cell RNA sequencing on 14,750 XX and XY mouse germ cells between embryonic days 10.5 and 16.5. By computational gene regulation inference analysis, we identified sex-specific, sequential waves of master regulator genes during germ cells differentiation and unveiled that the meiotic initiator Stra8 is regulated by positive and negative master regulators acting in an antagonistic fashion. Consistent with the importance of the somatic environment, we found that rare adrenal germ cells exhibit delayed meiosis and display altered expression of master genes controlling the female and male genetic programs. Our study provides a molecular roadmap of germ cell sex determination at single-cell resolution will serve as a valuable resource for future studies of gonad development, function and disease. SOURCE: Chloe MAYERE (chloe.mayere@unige.ch) - UNIGE
View on GEOView in PlutoEnhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreUse Pluto's intuitive interface to analyze and visualize data for this experiment. Pluto's platform is equipped with an API & SDKs, making it easy to integrate into your internal bioinformatics processes.
Read about post-pipeline analysisView quality control data and experiment metadata for this experiment.
Request imports from GEO or TCGA directly within Pluto Bio.
Chat with our Scientific Insights team