PLX297782

GSE136910: RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: to profile transcriptome changes due to EC-AGO1-deficiency; Methods: Subcutaneous adipose tissue were harvested. RNA was extracted using TRIzol and library was prepared for sequencing.; Results: We found genes involved in pathways promoting angiogenesis, browning, insulin sensitivity to be upregulated, and those promoting inflammation and fibrosis to be decreased in KO compared with WT mice.; Conclusions:Our study represents the detailed analysis of Ago1 regulate transcriptomes in SAT between WT and KO mice. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. SOURCE: Zhen Chen (zhenchen@coh.org) - Dr. Zhen Chen Lab City of hope