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Learn MoreTo determine how the transcription factors GR and STAT3 cooperate to regulate gene expression, the MDA-MB-231 breast cancer cell line was treated with dexamethasone (1M for 24 hours) and the STAT3 inhibitor SH4-54 (8M for 24 hours) alone and in combination. A multivariate linear model with a GR:STAT3 interaction term was used to identify genes that are differentially expressed when both TFs are fully active in the nucleus. SOURCE: K-T Varley University of Utah
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