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Learn MorePurpose: to investigate the role of transcription factor Foxp1 in murine A20 lymphoma cells, which can be used as a model for human diffuse large B cell lymphoma; Method: Exons 6 and 7 of Foxp1 were independently targeted using CRISPR/Cas9 editing, and 2 clones from each targeted exon (E6 and E7), showing depleted Foxp1 isoforms were isolated.; Method: RNA sequencing was conducted on parental A20 cells and CRISPR clones, and differential gene expression was undertaken SOURCE: Ling Felce (ling.felce@ndorms.ox.ac.uk) - University of Oxford
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