PLX288185

GSE139907: Genome wide gene expression profiling and methylation profiling of directly reprogrammed osteoblasts from mouse fibroblasts

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

We identified four transcriptional factors, Runx2, Osx, Dlx5, and ATF4, that rapidly and efficiently reprogram mouse fibroblasts derived from 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP) transgenic mice into induced osteoblast cells (iOBs). The global transcriptome profiling validated that iOBs resemble primary osteoblasts. Genome-wide DNA methylation analysis demonstrates that within differentially methylated loci, the methylation status of iOBs more closely resembles primary osteoblasts than mouse fibroblasts. We further demonstrate that Col2.3GFP+ iOBs have transcriptome profiles similar to GFP+ cells derived from Col2.3GFP transgenic mouse bone chips. SOURCE: Hui Zhu (huizhu@stanford.edu) - Stanford Universtiy