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Learn Morebulk sequencing outputs of Caco2 cells after exposure to permeability modifying and permeability rescuing agents.; To identify the molecular drivers for methotrexate-induced barrier dysfunction, we conducted RNA sequencing on Caco2 spheroids treated with methotrexate and lactoferrin. Given our observation that barrier function was compromised as early as 4-6 hours after exposure to methotrexate, we isolated RNA from spheroids 4-hour post-treatment to capture the transcriptional events responsible for initiating the processes. SOURCE: Thomas Wallach (thomas.wallach@gmail.com) - Gartner UCSF
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