PLX302525

GSE140109: Myeloid-derived suppressor cells facilitie invasion and metastasis of papillary thyroid cancer cells by repressing miR-486-3p

• Organsim human
• Type RNASEQ
• Target gene
• Project ARCHS4

BackgroundIn present study, we aimed to evaluate the level as well as function of circulating MDSCs in papillary thyroid cancer (PTC), and also explore theunderlyingmechanismof MDSCs during tumor progression.; MethodsmicroRNA arrays and RNA-seq analysis were conducted to screened out differentially expressed miRNAs and mRNAs between TPC-1 cells cocultured with PMN-MDSCs and untreated TPC-1 cell line.; Results19 miRNAs were differentially expressed, of which 10 were downregulated and 9 were upregulated in MDSC-conditioned cancer cells. GO analysis of miRNA predicted target genes showed that inflammatory related response were enriched. Besides, these genes were mainly related to NF-kappa B, PI3K-AKT and TNF signaling pathways which are associated with immune response as well as cancer development by KEGG pathway analysis. For validation, qRT-PCR was used to assess the differentially expressed miRNA and the trends in the expression was consistent between microRNA-seq and qRT-PCR.; ConclusionCollectively, our work explore the mechanism by which PMN-MDSCs paly a critical role in PTC SOURCE: Li Chen (chenli73@mail2.sysu.edu.cn) - The First Affiliated Hospital of Sun Yat-sen University