Key Features
Enhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreBackground: Maternal enviromnetal exposures during pregnancy can transplacentally train the developing fetal immune system. We have proviously shown that treatment of pregnant mice with the microbial-derived OM-85 can protect their offspring against allergic airways disease.; Purpose: To characterise the cellular/molecular mechanisms underpinning this protection, focusing on the fetal bone marrow as a potential mechanistic target.; Methods: Pregnant mice were orally treated with OM-85 from gestation day (GD) 9.5-17.5. Fetal bone marrow and thymus was collected from OM-85 treated and non-treated mothers at GD18.5. Cellular and molecular profiles were characterised by multi-colour flow cytometry and transcriptomics (RNA-Seq) respectively.. Molecular profiling utilised upstream regulator analysis .Protein-level validation of upstream regulator analysis results was performed by multi-colour flow cytometry.; Results: Maternal OM-85 treatment induces transplacental signals that manifest in fetal bone marrow as an enriched population of conventional dendric cells (cDC) displaying enhanced functional maturation. Moreover, the myeloid progenitor populations directly upstream of this cDC pool were significantly boosted in response to maternal treatment. Transcriptomic analysis of fetal bone marrow identified maternal OM-85-induced activation of X-box binding protein 1 (XBP1), with upregulation of active XBP1 localised to cDC precursors.; Conclusions: Maternal OM-85 treatment during pregnancy transplacentally accelerates functional immunocompetence of fetal bone marrow cDCs and for the first time we identify the transcription factor XBP1 as a putative driver of this immune training mechanism. SOURCE: Kyle Mincham (kyle.mincham@telethonkids.org.au) - Telethon Kids Institute
View on GEOView in PlutoEnhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreUse Pluto's intuitive interface to analyze and visualize data for this experiment. Pluto's platform is equipped with an API & SDKs, making it easy to integrate into your internal bioinformatics processes.
Read about post-pipeline analysisView quality control data and experiment metadata for this experiment.
Request imports from GEO or TCGA directly within Pluto Bio.
Chat with our Scientific Insights team