PLX032540

GSE140554: RNAseq for the identification of genes induced upon TGR5 stimulation in intestinal stem cells

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: Transcriptomic exploration for the identification of genes induced upon TGR5 stimulation in intestinal stem cells; Methods: For each biological replicate, GFPhi cells were isolated by FACS from intestines from 4 pooled Lgr5-eGFP-IRES-CreERT2 mice. About 200.000 GFPhi cells were then embedded in Matrigel (20.000 per well in 10L Matrigel drop) and after 4 hours were treated with INT-777 (30M) or DMSO as control. After 16 hours RNA was extracted (RNAqueous Kit, Thermofisher) for RNAseq analysis. This experiment was repeated 3 times in 3 different days (n=3 biological replicates).; Results: INT-777 treatment results in the activation of a panel of genes showing high enrichment of TEADs family of TF in associated motifs. SOURCE: giovanni sorrentino (gaby.elalam@lau.edu, kristina.schoonjans@epfl.ch, giovanni.sorrentino@epfl.ch) - Schoonjans Lab – Laboratory of Metabolic Signaling École polytechnique fédérale de Lausanne