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Learn MorePurpose: Analyze the potential signaling pathways regulated by MLKL under TNF-induced endothelial cell inflammatory response; Methods: HUVECs were allowed to grow to confluency and were starved with basal medium containing 0.5% FBS for 6h. Then treat HUVECs with vehicle, NSA, TNF-alpha, TNF-alpha+NSA for 6h and extract RNA.; Results: NSA reduces the mRNA level of TNF-induced inflammation-related genes and most of these genes are downstream of NF-kappa B signaling pathway. SOURCE: Dai Jialin (daijialin@sioc.ac.cn) - Interdisciplinary Research Center of Biology and Chemistry
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