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Learn MoreTo investigate the effect of MGL ligation in monocyte-derived dendritic cell biology, we generated control dendrimers and two different glycodendrimers exposing the MGL ligands GalNAc or GalNAc1-4Gal. GalNAc and GalNAc1-4Gal glycodendrimers were validated in the corresponding manuscript. Monocyte-derirved dendritic cells were generated by culturing monocytes for 4 days with IL-4 and GM-CSF. To study the effect of MGL-ligation at the transcriptional level, next generation sequencing was performed on monocyte-derived dendritic cells from three independent donors, stimulated for 4 h with control, GalNAc or GalNAc1-4Gal glycodendrimers in the absence or presence of LPS. SOURCE: Anouk Zaalvan Vliet Amsterdam UMC
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