Key Features
Enhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreThe IgH 3 regulatory region (3RR) controls class switch recombination (CSR) and somatic hypermutation (SHM) in B cells. The mouse 3RR contains four enhancer elements with hs1,2 flanked by inverted repeated sequences and the center of a 25-kb palindrome bounded by two hs3 enhancer inverted copies (hs3a and hs3b). hs4 lies downstream of the palindrome. Evolution maintained in mammals this unique palindromic arrangement suggesting that it is functionally significant. We report that deconstructing the palindromic IgH 3RR strongly impacts its function even when enhancers are preserved. CSR and IgH transcription appear poorly dependent from the 3RR architecture and are more or less preserved provided 3RR enhancers are present. By contrast, an architectural effect significantly lowers VH germline transcription, AID recruitment and SHM. In conclusion, this work indicates that the IgH 3RR does not simply pile up enhancer units but also optimally expose them into a functional architecture of crucial importance. SOURCE: Kevin LebrigandFunctional Genomics Platform of Nice-Sophia-Antipolis, France. IPMC/CNRS
View on GEOView in PlutoEnhance your research with our curated data sets and powerful platform features. Pluto Bio makes it simple to find and use the data you need.
Learn MoreUse Pluto's intuitive interface to analyze and visualize data for this experiment. Pluto's platform is equipped with an API & SDKs, making it easy to integrate into your internal bioinformatics processes.
Read about post-pipeline analysisView quality control data and experiment metadata for this experiment.
Request imports from GEO or TCGA directly within Pluto Bio.
Chat with our Scientific Insights team