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Learn MoreDuring giNPC induction process, cell samples were harvested at various induction days including named Day1, Day4, Day7, Day10, Day14, Day17 and Day21. Meanwhile, samples from primary neurosphere-like cluster (named pre-NPCs), established giNPC cell line at passage 6 (named giNPCs P6), Brain-NPCs as well as Teratoma were also collected. Total RNA was isolated from cell pellets by Trizol reagent and RNA-Seq library were generated using KAPA Strandard mRNA-Seq Kits according to the manufacturer's recommendations. SOURCE: Shaorong Gao (gaoshaorong@tongji.edu.cn) - Tongji University
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