PLX315127

GSE95835: Transcriptome of choroid endothelial cells from P5 and P30 mice.

• Organsim mouse
• Type RNASEQ
• Target gene
• Project ARCHS4

Purpose: The outer blood-retina barrier is established through the coordinated terminal maturation of the retinal pigment epithelium (RPE), fenestrated choroid endothelial cells (ECs) and Bruchs membrane, a highly organized basement membrane that lies between both cell types. Here we study the contribution of choroid ECs to this process by comparing their gene expression profile before (P5) and after (P30) the critical postnatal period when mice acquire mature visual function.; Methods: ECs from P5 and P30 mice were labeled in vivo by retro-orbital injection of fluorescently-labeled anti-VE-Cadherin. After 10 minutes, mice were euthanized, eyeballs were enucleated and the anterior segment was discarded. After removal of the neural retina, RPE/choroid was mechanically dissected from the sclera and digested. ECs were isolated by flow cytometry and processed immediately for RNA extraction.; Results: Transcriptome analyses show that whereas P5 choroid EC transcriptome is preferentially enriched in cell cycle- and chromosome-related transcripts, reflecting an immature phenotype, the transcriptome of adult (P30) choroid ECs is enriched in genes encoding proteins involved in biological adhesion, including a variety of extracellular matrix (ECM)-related genes.; Conclusion: these results strongly suggest that mature choroid ECs actively participate in extracellular matrix assembly and regulation. SOURCE: Ignacio BenedictoRodriguez-Boulan Weill Cornell Medical College